Fig. 3
From: The CB1 cannabinoid receptor regulates autophagy in the tibialis anterior skeletal muscle in mice
Knockout CB1 prevents LC3 II accumulation in tibialis anterior muscle. p62/Sqstm1, LC3, AKT473, total AKT, mTOR2448, total mTOR, p70S6K389, total p70S6K, AMPK172, and total AMPK protein levels were determined by western blot. A Representative western blot image. B p62/Sqstm1 protein levels. C LC3 I protein levels. D LC3 II normalized by total LC3. E Representative western blot image. F AKT473. G mTOR2448. H p70S6K389. I AMPK172. Two-way analysis of variance (ANOVA) with Bonferroni’s post hoc test. Statistical significance was set at p < 0.05. Values are expressed as mean and S.E.M. and scatter dot plots, as appropriate