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Fig. 2 | Biological Research

Fig. 2

From: The pattern of alternative splicing and DNA methylation alteration and their interaction in linseed (Linum usitatissimum L.) response to repeated drought stresses

Fig. 2

Identification of drought stress-responsive AS events in linseed seedlings. a The number of drought stress-responsive AS events identified in Z141 and NY-17 under the DS and RD treatments. b The number of differentially spliced genes (DSGs) identified in Z141 and NY-17 under the DS and RD treatments. c Bubble plots showing examples of Z141 and NY-17 GO enrichment under different drought stresses. the bubble size indicates the number of enriched genes. The complete GO analysis results are provided in Additional file 5. d Clustering analysis of drought stress-responsive AS events was performed based on changes in isoform expression percentage (IEP) after the DS, RW and RD treatments. According to the changes in IEP between the control and each stress condition, the drought stress responses to AS events were clustered into 6 groups. The numbers in the figure indicate the number of AS events for the corresponding cluster. The abscissa indicates different drought stress treatments, and the ordinate indicates the normalized ΔIEP value. The darker line in each graph indicates the average expression trend. e, f GO terms significantly enriched by DSGs of Z141e and NY-17 f in Cluster 1

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