Fig. 4

DYRK1 is an endocytosis regulator. A KEGG analysis of the phosphorylation level of the downregulated phosphoproteins. The significantly enriched 30 high-ranking KEGG pathways indicated that endocytosis was regulated by DYRK1. B The phosphorylation levels of some critical endocytic components and exocytotic components were downregulated. C The loss of function of DYRK1 by chemical inhibitors disturbed endocytosis. The labeled dextran in a punctate pattern was obviously observed in DMSO treatment embryos, while a relatively weak signal was found in DYRK1 inhibitor treatment embryos (AZ191, 40 μM; Harmine, 40 μM). A white dotted-line depicts edges of the magnified embryos. Scale bar in whole embryo panel, 100 μm; scale bar in magnification panel, 20 μm