Fig. 3

DYRK1-mediated phosphorylation of endophilin is required for Ciona notochord lumen expansion. A Schematic representation of the mutant constructs of endophilin (deletion of residues 263–356 or amino acid substitution at S263A). B–C Overexpression of endophilin-N (1–262) arrested lumen expansion significantly. D–E Overexpression of endophilin (S263A) arrested lumen expansion significantly. The white circle depicts the edge of the lumen, and the white asterisk indicates lumen with phenotype, and the red asterisk indicates control lumen. “n” represents the statistical number of the lumens. Asterisks (***) represent statistical significance (p < 0.001). Scale bar 10 μm