Fig. 8
From: Disseminated intravascular coagulation phenotype is regulated by the TRPM7 channel during sepsis
TRPM7 regulates endotoxin-induced PNAs formation an adhesion to endothelial cells. Representative images from vehicle- and endotoxin-treated ECs non-transfected (A), transfected with a siRNA against TRPM7 (siRNATRPM7) (B) or transfected with a siRNA control (siRNANontarget) (C) cocultured with platelets for 24 h, and then, with neutrophils for 30 min. Platelets were stained by Vybrant Dio (green) and neutrophils by Vybrant Dio (red) and the level of adhesion of PNAs (D), platelets (E), neutrophils (F) to ECs was analyzed (N = 5). Bar scale represents 100 μm. Relative adhesion to ECs of PNAs, platelets and neutrophils in the triculture (G). PNAs aggregates on ECs were determined by close or merged green/red staining over the EC monolayer depicted by the arrows. Results were normalized against vehicle-exposed non-transfected cells (control condition). Statistical differences were assessed by a two-way analysis of variance (ANOVA) followed by Tukey post hoc test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001, compared with the vehicle-treated condition in the saline- or non-transfected-condition. Results showed as mean ± SEM