Fig. 6
From: Disseminated intravascular coagulation phenotype is regulated by the TRPM7 channel during sepsis
TRPM7 regulates endotoxin-induced translocation of NF- κB into the nucleus in endothelial cells. Representative images from vehicle- and endotoxin-treated ECs exposed to saline solution (A), carvacrol (B), 2-APB (C), or non-transfected (E), transfected with a siRNA against TRPM7 (siRNATRPM7) (F) or transfected with a siRNA control (siRNANontarget) (G) for 24 h, and then NF-κB (green) was detected by immunofluorescence. Nuclei were stained using DAPI. Bar scale represents 50 μm. Quantification of NF-κB (D and H) was performed by densitometric analysis (N = 3). Data were expressed as the normalized ratio of NF-κB signal (pixels/μm2) in nucleus/cytosol. Results were normalized against vehicle-exposed cells (control condition). Statistical differences were assessed by a two-way analysis of variance (ANOVA) followed by Tukey post hoc test. ****p < 0.0001, compared with the vehicle-treated condition in the saline- or non-transfected-condition. Results showed as mean ± SEM