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Fig. 5 | Biological Research

Fig. 5

From: Disseminated intravascular coagulation phenotype is regulated by the TRPM7 channel during sepsis

Fig. 5

TRPM7 modulates endotoxin-induced vWF, ICAM-1 and P-Sel expression in endothelial cells. Representative images from vehicle- and endotoxin-treated ECs exposed to saline solution (A), carvacrol (B), 2-APB (C), or non-transfected (G), transfected with a siRNA against TRPM7 (siRNATRPM7) (H) or transfected with a siRNA control (siRNANontarget) (I) for 24 h, and then vWF, ICAM-1 and P-Sel expression was detected by immunofluorescence. Bar scale represents 50 μm. Quantification of protein expression of vWF (D and J), ICAM-1 (E and K) and P-Sel (F and L) was performed by densitometric analysis (N = 5). Results were normalized against vehicle-exposed cells (control condition). Statistical differences were assessed by a two-way analysis of variance (ANOVA) followed by Tukey post hoc test. ***p < 0.001 and ****p < 0.0001, compared with the vehicle-treated condition in the saline- or non-transfected-condition. Results showed as mean ± SEM

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