Fig. 3

TRPM7 inhibition protects mice from increased neutrophil-endothelial interaction (NEI) during endotoxemia. A NEI in mice treated with LPS 3 mg/kg i.p. and or carvacrol 80 mg/kg i.p. (1 h post-endotoxemia) were inspected using a scanning laser ophthalmoscope (SLO) to obtain confocal images of the fundus from the left eye. B–E Representative SLO images obtained from a real-time movie depicting NEI count at 3 h (B) and 12 h (C) post-endotoxemia. Representative SLO images obtained from a real-time movie depicting NEI count at 3 h (D) and 12 h (E) post-endotoxemia in mice subjected to injection of carvacrol 1 h after endotoxemia. F Serial quantification of SLO captures from endotoxic mice in the absence or presence carvacrol at 0,3, 6, 12, 24, 48 and 72 h post-endotoxemia. Grey: reflectance with 638 nm laser. Red: rat anti-Ly6G. Arrowheads: neutrophils labelled with an anti-Ly6G IgG. v: Venules. a: Arterioles. n: Optic nerve head. Scale bar = 100 μm. (N = 5 per group). Statistical differences were assessed by two-way ANOVA followed by Dunnett's post hoc test. **p < 0.01, ****p < 0.0001 comparing endotoxemic (red) vs endotoxemic/carvacrol (green) conditions. Results showed as mean ± SEM