Fig. 12

High TRPM7 expression increases in CMECs and CEPCs from SSPs, which correlates with DIC and survival time. A Survival (Kaplan–Meier) curves comparing SSPs exhibited a DIC-Score ≥ 5 (red line) with SSPs exhibited a DIC-Score < 5 (soft-red line). *, p = 0.0001 (log-rank (Mantel–Cox) test). ¶, p = 0.0002 (Gehan-Breslow-Wilcoxon test). B Percentage of SSPs exhibiting a DIC-Score ≥ 5 or DIC-Score < 5 showing high (red) and low (green) TRPM7 mRNA and protein expression in their CMECs and CEPCs. Survival (Kaplan–Meier) curves comparing high (solid soft-blue and soft-green) and low (dotted blue and green) TRPM7 mRNA and protein expression in CMECs (C) and CEPCs (D) with SSPs survival time. *, p = 0.0001 and #, p = 0.003 (log-rank (Mantel–Cox) test). ¶, p = 0.0002 and §, p = 0.007 (Gehan-Breslow-Wilcoxon test). Contingency analyses performed to determine relative risk between high (soft-blue and soft-green) and low (blue and green) TRPM7 mRNA and protein expression in CMECs (E) and CEPCs (F) respect to showed in C and D, respectively. ‡, p < 0.05, compared with the total (high and low together) mRNA and protein expression level of TRPM7. AUROC curve analysis in CMECs (G) and CEPCs (H) extracted from SSPs were performed for TRPM7 mRNA and protein expression. AUC and p-value score are: CMECs: AUC = 0.8625, p = 0.0022 (95% CI 0.7199–0.999) for TRPM7 mRNA expression and AUC = 0.9010, p = 0.0006 (95% CI 0.7702–1.000) for TRPM7 protein expression. CEPCs: AUC = 0.7857, p = 0.0290 (95% CI 0.5925–0.9789) for TRPM7 mRNA expression and AUC = 0.7531, p = 0.0350 (95% CI: 0.5638–0.9424) for TRPM7 protein expression. APACHE II: AUC = 0.5045, p = 0.9728 (95% CI: 0.2596–0.7493). SOFA: AUC = 0.5413, p = 0.7427 (95% CI 0.2934–0.7892). Diagonal discontinuous line in plots is the non-discrimination line. The best sensitivity (S) and specificity (E) to predict mortality outcome in SSPs and the corresponding Youden INDEX (YI) are: TRPM7 mRNA expression in CMECs: S: 90,00%, E:75,00%, YI:0.68, cut-off ≥ 2.698; TRPM7 protein expression in CMECs: S:83.33%, E:90.00%, YI:0.67, cut-off ≥ 2.299; TRPM7 mRNA expression in CEPCs: S:71,43%, E:87,50%, YI:0.61, cut-off ≥ 2.947; TRPM7 protein expression in CEPCs: S:72.22%, E:77.78%, YI:0.59, cut-off ≥ 1.825; APACHE-II: S:50.00%, E:62.50% and YI:0.28, cut-off ≥ 22.7 and SOFA: S:54.55%, E: 54.55% and YI:0.32, cut-off ≥ 8.3