Fig. 4

EPC-EXs.^miR-17-5p induced inhibition of SPRED1 and activation of the PI3K/Akt signaling pathway in Hypoxia plus HG-injured ECs or C2C12. A Immunofluorescence images of PKH26 labeled EPC-EXs merged into ECs and C2C12. B The levels of miR-17-5p in ECs and C2C12 after incubation with different EPC-EXs. C SPRED1 is a potential target of miR-17-5p. D The levels of SPRED1, PI3K, and p-Akt/Akt in ECs and C2C12 after incubation with different EPC-EXs (B One-way ANOVA, followed by Tukey’s post hoc test; D Two-way ANOVA, followed by Tukey’s post hoc test. Data represent the mean ± SEM. n = 3/group)