Fig. 2
EPC-EXsmiR-17-5p infusion increases miR-17-5p levels, angiogenesis, blood perfusion, and MVD in hind limb of DHI mice. A Images showing PKH 26 labeled EPC-EXs (red) merged into vascular ECs (CD31, green). Scale bar, 30 μm. B The levels of miR-17-5p in hind limb vessels after EPC-EXs and EPC-EXsmiR-17-5p administration. C Representative images and summary data of angiogenesis in hind limb tissue of DHI mice. D Representative images and summary data of blood perfusion in hind limb tissue of DHI mice. E Representative images and summary data of MVD (CD31, red; DAPI, blue) in hind limb tissue of DHI mice. *p < 0.05 compared with the Sham group; #p < 0.05 compared with PBS group; +p < 0.05 compared with EPC-EXs group (B, D, E Two-way ANOVA, followed by Tukey’s post hoc test; C One-way ANOVA, followed by Tukey’s post hoc test. Data represent the mean ± SEM, n = 8 mice per group)