Fig. 1

MSCs differentiation to osteoblasts and adipocytes. A Three donors MSCs differentiated to osteoblasts (21 days) and adipocytes (14 days) were stained with alizarin red and oil red, respectively. B Expression of osteoblast marker genes in MSCs differentiated to osteoblasts. C Expression of adipocyte marker genes in MSCs differentiated to osteoblasts. All the experiments were performed at least in 3 different donors. Data is expressed as mean ± SEM. * p < 0.05, **p < 0.01. All gene expression data were normalised to the reference gene, GAPDH, and expressed as fold relative to the control samples