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Fig. 7 | Biological Research

Fig. 7

From: Alpha‐kinase1 promotes tubular injury and interstitial inflammation in diabetic nephropathy by canonical pyroptosis pathway

Fig. 7

HK-2 cells were treated with ALPK1 siRNA or non-target siRNA for 6 h prior to 24 h-HG (30 mM) treatment. A Representative images of immunofluorescence staining for GSDMD (green) with nuclei marked by DAPI (blue) show knockdown of ALPK1 inhibited translocation of GSDMD to the cell membrane (arrow shows) induced by high glucose (scale bar, 50 μm; magnification ×400). B The protein levels of IL-1β and IL-18 in HG-treated HK-2 cells were determined by western blot. C IL-18 level in supernatant of HK-2 cells was measured by ELISA. Experiments were performed in triplicate. Data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (compared with LG group). #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 (compared with HG group)

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