Fig. 6

HK-2 cells were treated with ALPK1 siRNA or non-target siRNA for 6 h prior to 24 h-HG (30 mM) treatment. A Representative images of immunofluorescence staining for NF-κB P65 (green) with nuclei marked by DAPI (blue). ALPK1 knockdown inhibited the nuclear translocation of NF-κB P65 (arrow shows) (scale bar, 50 μm; magnification ×400). B, B1 The protein level of phospho-NF-κB P65 in four groups were determined by western blot. C, C1 Western blot determined cellular distribution of P65 in HK-2 cells. Experiments were performed in triplicate. Data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (compared with LG group). ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 (compared with HG group)