Fig. 5

HK-2 cells were treated with ALPK1 siRNA or non-target siRNA for 6 h prior to 24 h-HG (30 mM) treatment. A Densitometry analysis shows knocking down ALPK1 using siRNA transfection downregulated ALPK1 (A1), caspase-1 (A2), caspase-1 P20 (A3), GSDMD (A4), GSDMD-NT (A5) and α-SMA (A6) which increased under high glucose environment. B, B1 Flow cytometry analysis shows high glucose (5.63%) increased proportion of pyroptotic HK-2 cells compared with the control (3.41%) and the proportion was reversed after knockdown of ALPK1 (3.98%). Quadrant 2 presented ratio of pyroptosis cells. Experiments were performed in triplicate. Data were presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 (compared with LG group). ^#P < 0.05, ^##P < 0.01, ^###P < 0.001, ^####P < 0.0001 (compared with HG group)