Fig. 4

A, A1 The protein levels of ALPK1 in HK-2 cells treated with 5.5, 10, 20 or 30 mM d-glucose for 24 h were determined by western blot. B, B1 The protein levels of ALPK1 in HK-2 cells treated with 30 mM d-glucose for different periods of time (0, 12, 24 or 48 h) were determined by western blot. Experiments were performed in triplicate. Data were presented as mean ± SD (ns no significance, **P < 0.01, ****P < 0.0001)