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Fig. 6 | Biological Research

Fig. 6

From: Ubiquitin protein E3 ligase ASB9 suppresses proliferation and promotes apoptosis in human spermatogonial stem cell line by inducing HIF1AN degradation

Fig. 6

ASB9 inhibited SSC proliferation by regulating HIF1AN. A, B Western blot analysis was performed to confirm transfection efficiency, and the results showed that HIF1AN expression plasmid re-expressed HIF1AN protein in ASB9 overexpression SSCs. C CCK8 was used to detect cell proliferation after HIF1AN was re-expressed in ASB9 overexpression SSCs. D, E Western blot showed the protein level of PLZF and PCNA after transfection with HIF1AN expression plasmid in ASB9 overexpression SSCs. (F-G) Cellular DNA synthesis was detected by EdU analysis. Scale bar, 20 μm. (H-I) Cell apoptosis analysis after re-expressed HIF1AN using Flow Cytometry. * indicates significant difference (p < 0.05) and ** indicates extremely significant difference (p < 0.01)

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Biological Research

ISSN: 0717-6287

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