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Fig. 5 | Biological Research

Fig. 5

From: Ubiquitin protein E3 ligase ASB9 suppresses proliferation and promotes apoptosis in human spermatogonial stem cell line by inducing HIF1AN degradation

Fig. 5

ASB9 downregulated HIF1AN in human SSC line. A Prediction of ASB9-interacting proteins by GeneMANIA, STRING and HitPredict. B The Violin plot showed the expression of HIF1AN, CKB, CKM and CKMT1B in testicular cells. C, D Western blot revealed that ASB9 overexpression led to a reduction of HIF1AN and CKB. E Immunoprecipitation demonstrates the interaction of ASB9 with HIF1AN. * p < 0.05 indicates significant differences between pCMV3 and pCMV3-ASB9 groups

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