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Fig. 3 | Biological Research

Fig. 3

From: Ubiquitin protein E3 ligase ASB9 suppresses proliferation and promotes apoptosis in human spermatogonial stem cell line by inducing HIF1AN degradation

Fig. 3

The influences of ASB9 overexpression on human SSC line proliferation. A, B Western blot showed the level of ASB9 in the human SSC line after transfected with pCMV3 and pCMV3-ASB9 plasmid. C CCK-8 assay displayed the proliferation of human SSC line transfected with pCMV3 and pCMV3-ASB9 plasmid. D, E Percentages of EdU-positive cells after transfection of pCMV3 and pCMV3-ASB9 plasmid. F, G Western blot showed the protein level changes of PLZF, CCNE1, PCNA, and THY1 after transfection with a pCMV3-ASB9 plasmid, ACTB protein served as the loading control. Scale bars in D, 50 μm. *Indicates significant difference at p < 0.05, ** indicates significant difference at p < 0.01, *** Indicates significant difference at p < 0.001

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Biological Research

ISSN: 0717-6287

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