Fig. 2

The expression of ASB9 in human testis. A Western blot showed the level of ASB9 in three testes of OA samples with normal spermatogenesis, ACTB was used as the loading control of total protein. B Representative H&E image of OA testicular tissues. Scale bars,200 μm. C Immunohistochemistry images for cell localization of ASB9 in OA sample. Scale bars, 50 μm. D Double immunostaining revealing co-expressions of ASB9 with UCHL1, GFRA1, KIT, and PCNA in human testis with normal spermatogenesis. Scale bars, 50 μm. E Percentages of ASB9⁺ cells co-expressing UCHL1, GFRA1, PCNA, and KIT. At least 20 seminiferous tubules were assessed. Each circle represents a count result, and the dashed line in the figure shows the mean value of the 20 count results