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Fig. 1 | Biological Research

Fig. 1

From: Ubiquitin protein E3 ligase ASB9 suppresses proliferation and promotes apoptosis in human spermatogonial stem cell line by inducing HIF1AN degradation

Fig. 1

Human spermatogonia stem cell profiling based on scRNA-seq analysis. A UMAP and clustering analyses of testis single-cell transcriptomic data from GSE109037 and GSE120508. Each dot represents a single cell and is colored according to the identity of the cluster in the figure. B Reclustering analysis of SSCs cluster, SSCs were subgrouped into five stages and colored according to the legend. C Pseudo-time analysis on SSCs. The black line in the figure shows the cell developmental trajectory, and character 1 represents the starting point of the developmental trajectory. D Pseudo-time analysis on Top 10 differentially expressed genes at different stages. The x-axis denotes pseudo-time (defined in D). and the y-axis represents gene expression levels. The black curves in the graph showed the average expression level of the specified gene in all SSCs accompanying the developmental trajectory. E The Violin plot showed the expression pattern of the Top 10 differentially expressed genes in all testicular cells. UMAP: uniform manifold approximation and projection, SSCs: spermatogonial stem cells, Diff.ing Spg: differentiating spermatogonia, L: leptotene spermatocytes, L/Z: leptotene/zygotene spermatocytes, P: pachytene, D: diplotene spermatocytes, Late Spc: late spermatocytes, RS: round spermatids, ES: elongated spermatids, LCs: Leydig cells, SCs: Sertoli cells, PTM/LCs: peritubular myoid cells/Leydig cells, Mø: macrophages

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