Fig. 2
From: Oocyte quality assessment in marine invertebrates: a novel approach by fluorescence spectroscopy
Egg quality assessment in mussel and sea urchin. In the left and middle columns, there are representative fluorescence emission spectra recorded by spectrofluorimetry in the Mytilus galloprovincialis and Paracentrotus lividus eggs stained with: SYBR-14/Propidium iodide (PI) for viability assessment; JC-1 for mitochondrial membrane potential (MMP) determination; H2DCFDA and hydrogen peroxide for intracellular ROS level evaluation; and C11 BODIPY.581/591 for plasma membrane lipid peroxidation (LPO) estimation. For each fluorochrome, eggs have been divided in untreated and treated with: glutaraldehyde for SYBR14/PI; peroxidation promoters (ferrous sulfate and vitamin C) for C11-BODIPY581/591; hydrogen peroxide for intracellular ROS; carbonyl cyanide m-chlorophenyl hydrazone (CCCP) for JC-1 (positive controls). In the right column, mean values (± SD) of viability, MMP, intracellular ROS levels, and LPO recorded in untreated and treated eggs of the mussel (M. galloprovincialis) and the sea urchin (P. lividus and A. lixula) were compared. * indicates statistically significant differences (P ≤ 0.05)