Fig. 1
From: Oocyte quality assessment in marine invertebrates: a novel approach by fluorescence spectroscopy
Representative fluorescence images of sea urchin eggs stained with: SYBR-14/Propidium iodide (PI) for viability assessment; JC-1 for mitochondrial membrane potential determination; H2DCFDA for intracellular ROS level evaluation; and C11-BODIPY581/591 for plasma membrane lipid peroxidation estimation. For each fluorochrome, eggs have been divided in untreated (first row) and treated (second row) with: glutaraldehyde for SYBR14/PI; peroxidation promoters (ferrous sulfate and vitamin C) for C11-BODIPY581/591; hydrogen peroxide for intracellular ROS; carbonyl cyanide m-chlorophenyl hydrazone (CCCP) for JC-1 (positive controls). For all dyes, the merge image includes the green and the red channels, except for H2DCFDA dye, for which only the green channel has been showed. Scale bars 50 μm