Fig. 6
Binding of chloroplast-made (HE)3-DARPin G3 to HER2 on different cell lines by analytical flow cytometry. Binding was detected via His-tag specific and fluorescein isothiocyanate-coupled (FITC) antibodies. The fluorescence intensity logarithm of counted cells was plotted versus the cell count. Since all cell samples were homogeneous cell populations, the logarithm of the average fluorescence intensity was plotted as a single value for better comparison. Omission control cells were treated with antibodies in the absence of DARPin G3. Cells were incubated with PBS or anti-His-tag antibody followed by FITC conjugated antibody to serve as negative controls. Data from representative experiments are shown