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Fig. 5 | Biological Research

Fig. 5

From: The production of the first functional antibody mimetic in higher plants: the chloroplast makes the DARPin G3 for HER2 imaging in oncology

Fig. 5

Binding affinity of chloroplast-made DARPin G3 to the extracellular domain of HER2 measured by ELISA. Polystyrene 96 wells plate were coated with 100 μg of extracellular domain of HER2 and incubated with serially diluted chloroplast-made DARPin G3. An HRP-conjugated anti-His tag antibody was used for detection. Experiments were performed in triplicate and crude extract of isolated chloroplasts of wild-type plant and BSA served as a negative control

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