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Fig. 3 | Biological Research

Fig. 3

From: The production of the first functional antibody mimetic in higher plants: the chloroplast makes the DARPin G3 for HER2 imaging in oncology

Fig. 3

Molecular analysis of transplastomic plants. The transplastomic nature of the plants was confirmed by PCR analysis with a DARPin G3-specific primers (P1/P2) and b flanking sequence primers (P3/P4). M: 1 kb DNA sizer marker P: PRV-DARPin G3 plasmid as positive control; WT: wild-type plant; T1-3: three independent transplastomic plants; C¯: DNA free reaction as the negative control. c Southern blot confirms DARPin G3 gene integration and homoplasmy in three transplastomic plants. Transplastomic plants had one 6.5 kb fragment as expected for homoplasmic transformed plants, whereas the wild-type plant had a 4.5 kb fragment after DNA was digested with BglII. M: Dig-labeled molecular marker VII; WT: wild-type plant; T1-3: three independent transplastomic plants

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