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Fig. 2 | Biological Research

Fig. 2

From: The production of the first functional antibody mimetic in higher plants: the chloroplast makes the DARPin G3 for HER2 imaging in oncology

Fig. 2

Sequence, structure, and structural model of chloroplast-made DARPin G3 in complex with the HER2. a The chloroplast-made DARPin G3 N-terminal capping ankyrin repeat, two designed ankyrin repeat module, and C-terminal capping ankyrin repeat amino acid sequences. The secondary structure components (α-helices and β turns) are shown above their respective sequences. The designed ankyrin repeat modules consist of 26 defined framework residues and 7 randomized residues (6 potential interaction residues and 1 framework residue) shown in red. Also, the DARPin G3 has four mutations at defined framework positions, which are shown in blue. A (HE)3-tag for purification at the N-terminus and a cysteine containing linker at the C-terminus were underlined. b The 3D structure of DARPin G3 (PDB id: 2JAB), and c The modeling structure of chloroplast-made ((HE)3-tag was colored red, and cysteine-containing linker was colored orange). The backbones of the DARPin G3 are colored in cyan, which are superposed with the homology model of chloroplast-made DARPin G3 in yellow. d Structural model of the chloroplast-made DARPin G3 (yellow) in complex with the human epidermal growth factor receptor 2 HER2 (green). In magenta, the structure of trastuzumab Fab bound to HER2 is shown superimposed on the G3-HER2 complex

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