Fig.5

miR-1268a enhance invasion and migration capacity by inhibiting autophagy pathway. A BGI Database to determine miR-1268a predicted target genes and to analyze pathway enrichment of the predicted target genes. B LC3I/II and P62 protein expression in OL cells transfected with miR-1268a mimics or NC mimics were analyzed by WB, the right side is the grayscale analysis chart of WB (the ordinate is the target protein/GAPDH). C Fluorescence image of positive-LC3 fusion protein in OL cells transfected with miR-1268a mimics or NC mimics. Scale bar, 20 μm. D, E Transmission electron microscopy of autolysosomes in OL cells transfected with miR-1268a mimics or NC mimics (Primary lysosomes are marked with blue arrows and autolysosomes are marked with red arrows). Scale bar, 2 μm,1 μm. F Expression of some autophagy-related genes in OL cells transfected with miR-1268a mimics or NC mimics were detected by RT-qPCR. G LC3I/II and P62 protein expression in OE-miR-1268a-OL cells treated with DMSO and Rapamycin (10μΜ) were analyzed by WB, the right side is the grayscale analysis chart of WB (the ordinate is the target protein/β-actin). H, I The migration and invasion ability of OE-miR-1268a-OL and OE-NC-OL cells treated with autophagy activator rapamycin by Transwell analysis. (Three replicate wells were set in each group, which were repeated 3 times.10 random fields from three replicate Transwell were counted. n = 3) Scale bar, 64 μm. Data were expressed as the mean \(\pm\) SD, *p < 0.05, **p < 0.01, ***p < 0.001