Fig.4

miR-1268a promotes the metastasis of melanoma in vivo and in vitro. A miR-1268a expression levels in OL cells transfected with miR-1268a mimics or NC mimics were detected by RT-qPCR. B, C Overexpression of miR-1268a promoted the migration and invasion ability of OL cells as revealed by Transwell assay. (Three replicate wells were set in each group, which were repeated 3 times.10 random fields from three replicate Transwell were counted. n = 3) Scale bar, 64 μm. D miR-1268a expression levels in OL-SCs cells transfected with miR-1268a inhibitor or NC inhibitor were detected by RT-qPCR. E, F OL-SCs transfected with miR-1268a inhibitor or NC inhibitor and then isolated exosomes added to OL cells, the invasion and migration capacity of OL cells treated with OL-SCs-inhibitor-NC-EXO、OL-SCs-inhibitor-1268a-EXO and PBS was assessed by Transwell assay. (Three replicate wells were set in each group, which were repeated 3 times.10 random fields from three replicate Transwell were counted. n = 3) Scale bar, 64 μm. G The effect of miR-1268a on changes in OL cell cycle distribution was determined by flow cytometry. H The proportion of cells in OE-miR-1268a-OL cells and OE-NC-OL cells at different phase (G1, S, G2), The experiment was repeated 3 times for each group. I The proliferation capacity of OL cells transfected with miR-1268a mimics or NC mimics were measured by CCK-8. J miR-1268a expression levels in OL cells stably infected with miR-1268a-overexpressing lentivirus and NC lentivirus were detected by RT-qPCR. K Whole-lung fluorescent and bright images of lung metastasis in NOD-SCID mice after tail intravenous injection of OL cells treated with miR-1268a overexpressing or NC lentiviruses, n = 6. Scale bars, 1 mm. L H&E-stained images of mouse whole lung sections after NOD-SCID mice were stably infected with miR-1268a-overexpressing lentivirus and NC lentivirus OL cells by tail vein injection, scale bar, 2 mm. Scale bar for magnified image, 530 μm. Data were expressed as the mean \(\pm\) SD, *p < 0.05, **p < 0.01, ***p < 0.001