Fig.1From: Melanoma stem cells promote metastasis via exosomal miR-1268a inactivation of autophagyExosome secreted by melanoma stem cells promote metastasis of melanoma. A The morphology of the obtained exosomes (OL-SCs-EXO、OL-EXO) was analyzed by Transmission electron microscopy (TEM) Scale bar, 100 nm. B Size distribution and concentration of exosomes derived from OL cells and OL-SCs cells was performed by Nanoparticle Tracking Analysis (NTA). C The expression of typical exosome marker proteins(Alix, CD63, CD81, TSG101, Calnexin) was detected by Western blot. D, E OL-SCs-EXO (40 μg) promoted migration and invasion ability of OL cells as revealed by Transwell assay. (Three replicate wells were set in each group, which were repeated 3 times.10 random fields from three replicate Transwell were counted. n = 3) Scale bar, 64 μm. Data were expressed as the mean \(\pm\) SD, *p < 0.05, **p < 0.01, ***p < 0.001Back to article page