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Fig. 1 | Biological Research

Fig. 1

From: Characterization of metal(loid)s and antibiotic resistance in bacteria of human gut microbiota from chronic kidney disease subjects

Fig. 1

Selection and synthesis of primers for genetic determinants analysis. Primers were used to amplify resistance genes to metals and antibiotics in DNA isolated from stool samples. For antibiotics analysis, primers were obtained from the literature [30], later an alignment was carried out with all databases of MEGARes [31], CARD [32], and GenBank [33] and evaluated by “in silico” PCR from UGENE [34]. For metal(loid) resistance primer design, principal antibiotic resistance genes belonging to each 6 phyla of HGM were aligned to determine the consensus sequence. Primers were designed with Primer3 software [35, 36]

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