Table 2 Primers used in this study
Gene | Sequence | Tm | Amplicon (bp) | |
|---|---|---|---|---|
Cloning | ABH19_09590 | (F)ATGAACAACACTGCCGGACAC | 61 | 2469 |
(R)CTTTCCGTCCGGGCACATT | ||||
RT-PCR | ABH19_09575 | (F)GCGATGACAGGAAGAACCAT | 61 | 587 |
ABH19_09585 | (R)AATAAGTGGCGGGTTTGGAC | |||
ABH19_09585 | (F)CTGGTTCAGCAGTTTCTTTCG | 60 | 294 | |
ABH19_09590 | (R)GATTGACGGCCACAAAATTC | |||
ABH19_09590 | (F)ACCTCATCGAAAACGAATGG | 61 | 388 | |
ABH19_09595 | (R)GATCACCGCCTCTTTTCCAT | |||
ABH19_09595 | (F)GCTCGTTGTGTCCCGTCT | 60 | 392 | |
ABH19_09600 | (R)ACAATCGGCTTGCCAAAAT | |||
ABH19_09600 | (F)GTGCCGGAGGAGGAATTT | 60 | 286 | |
ABH19_09605 | (R)CAGACCGTAGACCGTTTCG | |||
RT-qPCR | ABH19_09585 | (F)ATCATCTACACGGGAGCCTTT | 60 | 110 |
ABH19_09585 | (R)GTCAGGGCAATGGAAAGAAGT | |||
ABH19_09590 | (F)ACAACTTCTGGAACGGATGG | 60 | 105 | |
ABH19_09590 | (R)GCCTCTTCGAGATCGTCTTTT | |||
ABH19_09595 | (F)ACCTGGAGTGTCTGGTCTGG | 60 | 111 | |
ABH19_09595 | (R)TGACCTCCTCCCAGTCTTTC | |||
ABH19_09600 | (F)GTCCCTGTTCTGTGCGATCT | 60 | 104 | |
ABH19_09600 | (R)CCGTTCGACAACAGGATTTT |