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Fig. 3 | Biological Research

Fig. 3

From: tiRNA-Val promotes angiogenesis via Sirt1–Hif-1α axis in mice with diabetic retinopathy

Fig. 3

The regulatory function of tiRNA-Val in HRMEC cells. a TaqMan RT-qPCR analysis of tiRNA-Val expression in HRMEC cells transfected with tiRNA-Val mimics, siRNA of tiRNA-Val and scramble sequence RNA. HRMEC cells transfected with scramble sequence RNA as control group. Data are represented as the mean ± SD, n = 10, p < 0.001 vs. control group. Statistical significance was assessed by two-tailed Student's t-test. b CCK-8 assay for HRMEC cells transfected with tiRNA-Val mimics, siRNA of tiRNA-Val and scramble sequence RNA. HRMEC cells transfected with scramble sequence RNA as control group. Data are represented as the mean ± SD, n = 9, p < 0.001 vs. control group. Statistical significance was assessed by two-tailed Student's t-test. c, d Migration assay for HERMEC cells transfected with tiRNA-Val mimics, siRNA of tiRNA-Val and scramble sequence RNA. e Detection of apoptosis by concurrent staining with Annexin V-FITC and PI. HRMEC cells transfected with tiRNA-Val mimics (left panel), siRNA of tiRNA-Val (middle panel) or scramble sequence RNA (right panel). Cells were subsequently stained with Annexin V-FITC conjugate and PI and were measured by flow cytometry. Live cells were both Annexin V and PI negative. At early stage of apoptosis, the cells bound Annexin V while still excluding PI. At the late stage of apoptosis, they bound Annexin V-FITC and stained brightly with PI

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