Fig. 4

Overexpression of neuronal GRK2 in spinal cord alleviated CFA-induced pain. A Experimental strategy for neuronal GRK2 overexpression. Neuron specific promoter hSyn targeted Cre-ERT2 recombination activated by Tamoxifen. B Tamoxifen-activated Cre-ERT2 upregulated the expression of GRK2 in the spinal cord. Results are normalized to GAPDH. n = 8 for AAV-eGFP group and AAV-GRK2 group. Data are expressed as means ± SEM. *p < 0.05. Student’s t test was used for comparisons. C The mechanical threshold to von Frey stimuli in the AAV-GRK2 mice was significantly increased as compared with that of the AAV-eGFP mice. n = 10 for AAV-eGFP group and AAV-GRK2 group. Data are expressed as means ± SEM. The statistical method is repeated measures ANOVA. *p < 0.05