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Fig. 6 | Biological Research

Fig. 6

From: Diosmetin inhibits cell proliferation and promotes apoptosis through STAT3/c-Myc signaling pathway in human osteosarcoma cells

Fig. 6

IL-6 treatment counteracted and wp1066 imitated the anti-proliferative activity of diosmetin in osteosarcoma Saos-2 and U2SO cells. Saos-2 or U2SO cells were treated with diosmetin with or without IL-6 for 24 h, replaced with fresh culture medium and cultured for 2 weeks, stained with crystal violet and photographed. A Representative images of colonies of Saos-2 and U2SO cells from six-well plates in which cells were treated with DMSO control, diosmetin or diosmetin + IL-6 using colony formation assay. B Quantification of colony number in DMSO-, diosmetin- or diosmetin + IL-6-treated Saos-2 and U2SO cells. C Representative images of colonies of Saos-2 and U2SO cells from six-well plates in which cells were treated with DMSO control, WP1066, diosmetin or WP1066 + diosmetin using colony formation assay. D Quantification of colony number in DMSO-, WP1066, diosmetin- or WP1066 + diosmetin—treated Saos-2 and U2SO cells. The results are shown as the mean ± SD of three independent experiments (n = 3, *P < 0.05, ***P < 0.001)

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