Fig. 3

Diosmetin induced apoptosis in osteosarcoma cells. A Flow cytometric analysis of apoptotic osteosarcoma cells. Saos-2 or U2SO cells were stained with Annexin V-FITC/PI after diosmetin (0, 10, 30 and 90 μM) treatment for 24 h and cell apoptosis were measured using flow cytometry. B and C Quantification of the percentage of apoptotic Saos-2 (B) and U2SO (C) cells after treated with diosmetin at various concentrations for 24 h. The results are shown as the mean ± SD of three independent experiments (n = 3, *P < 0.05, **P < 0.01 and ***P < 0.001 vs. DMSO control)