Fig. 5

Necroptosis is partly involved in IB3-1 cell death induced by FAC and erastin. Cells were treated with FAC (100 µM) and erastin (Eras) (10 µM) in the presence or absence of DFO (100 µM), NSA (10 µM), Nec-1s (50 µM), zVAD (50 µM), wortmannin (5 µM), NAC (10 mM) or β-mercaptoethanol (50 µM) for 12 h and cell viability was measured (A). MLKL membrane localisation was assessed by immunofluorescence. Representative images and quantification are shown (B). IL-6 and IL-8 mRNA levels were assessed (C). Scale bar represents 25 µm. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 for statistical analysis of the indicated groups