Fig. 3

Increased lipid peroxidation and iron levels in FAC and erastin treated IB3-1 cells. AECs were treated with FAC (100 µM) and erastin (Eras) (10 µM) in the presence or absence of DFO (100 µM) or Fer-1 (2 µM) for 8 h. Lipid peroxidation were assayed in cells using the lipophilic redox-sensitive dye C11-BODIPY 581/591, which shifts its fluorescence from red to green in response to oxidation. Representative images and quantification are shown (A). MDA (B) and ferrous iron levels (C) were assayed. Scale bar represents 400 µm. Data are expressed as mean ± SEM. **p < 0.01 and ***p < 0.001 for statistical analysis of the indicated groups