Fig. 1

IB3-1 cystic fibrosis AECs are susceptible to cell death caused by FAC and erastin. AECs were treated with FAC (100 µM) or erastin (Eras) (10 µM) and total iron was assayed (A). AECs were treated with FAC (50–400 µM) (B) and/or erastin (10 µM) (C) for 12 h and cell viability was assessed by MTS assay. Cells were treated with 100 µM FAC and 10 µM erastin for 12 h and LDH release was measured (D) or cells were imaged using IncuCyte Zoom instrument (E). Scale bar represents 150 µm. Data are expressed as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001 for statistical analysis of the indicated groups