Fig. 4

The effect of litreol on the redox states of cytochromes b₅₆₆ and b₅₆₂ in rat liver submitochondrial particles indicating that the electron flux is halted in between cytochromes b. Two milliliters of the reaction mixture were immediately stirred manually after each addition during the automatic wavelength change. a The antimycin-sensitive pre-steady-state reduction kinetics of cytochromes b₅₆₆ and b b₅₆₂ were monitored in submitochondrial particles dissolved in 1% DM of the 1.0 ml reaction medium. Ant, 5 µM antimycin A presence only; Ant + Litreol 5 µM antimycin A and 10 µM litreol. For other experimental conditions, see the “Methods” section. The results are representative of three independent experiments