Fig. 3
From: Antisense noncoding mitochondrial RNA-2 gives rise to miR-4485-3p by Dicer processing in vitro
In vitro-transcribed ASncmtRNA-2 is processed by recombinant Dicer in vitro. A Transcript was incubated with recombinant rDicer for 0–60 min at 37 °C, after which Northern blot was performed using probes P-AS2a, P-AS2b and P-AS2c (Additional file 4). Arrowhead: putative mature miRNA(s) generated by rDicer processing of the IR. B same as A but using probe P-4485-3p (Additional file 4). Arrow: pre-miR-4485; arrowhead: mature miR-4485-3p