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Baseline TAC phenotype
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|
Body weight (g)
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28.2 ± 0.1
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28.2 ± 0.2 (p = 0.13)
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29.2 ± 0.1 (a: p = 0.95; b: p = 0.18)
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|
Tibia length (mm)
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17.2 ± 0.05
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17.5 ± 0.03 (p = 0.89)
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17.2 ± 0.05 (a: p = 0.10; b: p = 0.12)
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|
Heart weight (mg)
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145.6 ± 1.0
|
201.4 ± 2.8 (p < 0.001)
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213.8 ± 2.8 (a: p < 0.001; b: p = 0.46)
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|
Heart weight/tibia length (mg/mm)
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8.5 ± 0.1
|
11.6 ± 0.1 (p < 0.001)
|
12.4 ± 0.2 (a: p < 0.001; b: p = 0.26)
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|
EDL mass/TL (mg/mm)
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0.77 ± 0.12
|
0.83 ± 0.05 (p = 0.14)
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0.81 ± 0.05 (a: p = 0.35; b: p = 0.61)
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|
Functional cardiac parameters
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|
LCCA velocity 2 weeks post TAC (mm/s)
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833.5 ± 15.3
|
333.0 ± 49.5 (a: p < 0.001; b: p = 0.90)
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|
RCCA velocity 2 weeks post TAC (mm/s)
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1148.7 ± 154.4
|
1524.1 ± 138.0 (a: p = 0.098; b: p = 0.42)
|
|
LVEF (%)
|
61 ± 5
|
46 ± 6 (p < 0.001)
|
47 ± 7 (a: p < 0.001; b: p = 0.76)
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|
FS (%)
|
32 ± 3
|
22 ± 4 (p < 0.001)
|
23 ± 4 (a: p < 0.001; b: p = 0.76)
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|
Diastolic LV volume (µL)
|
68.7 ± 5.58
|
103.9 ± 4.9 (p = 0.002)
|
107.1 ± 9.6 (a: p = 0.03; b: p = 0.77)
|
|
Systolic LV volume (µL)
|
34.6 ± 4.0
|
63.4 ± 5.5 (p < 0.001)
|
69.4 ± 10.9 (a: p = 0.007; b: p = 0.63)
|
|
Diastolic LV wall thickness (mm)
|
4.3 ± 0.1
|
4.7 ± 0.9 (p = 0.27)
|
4.4 ± 0.4 (a: p = 0.097; b: p = 0.69)
|
|
Systolic LV wall thickness (mm)
|
1.0 ± 0.1
|
1.1 ± 0.1 (p = 0.64)
|
1.1 ± 0.1 (a: p = 0.50; b: p = 0.82)
|
|
Histological evaluation of cardiac remodeling
|
|
Relative heart area
|
1
|
0.98 ± 0.05 (p = 0.72)
|
1.09 ± 0.03 (a: p = 0.022; b: p = 0.053)
|
|
Relative circumference
|
1
|
0.98 ± 0.02 (p = 0.45)
|
1.07 ± 0.02 (a: p = 0.01; b: p = 0.003)
|
|
Relative LV thickness
|
1
|
1.09 ± 0.08 (p = 0.40)
|
1.09 ± 0.06 (a: p = 0.23; b:p = 0.97)
|
|
Relative RV thickness
|
1
|
0.77 ± 0.08 (p = 0.044)
|
0.70 ± 0.06 (a:p = 0.004; b: p = 0.56)
|
|
Cardiomyocyte CSA (µm2)
|
50.3 ± 0.8
|
61.6 ± 1.2 (p < 0.001)
|
59.6 ± 1.0 (a: p < 0.001; b: p = 0.20)
|
|
Perivascular fibrosis (%)
|
1.8 ± 0.1
|
2.0 ± 1.1 (p = 0.75)
|
2.6 ± 0.2 (a:p = 0.27; b:p = 0.48)
|
|
Interstitial fibrosis (%)
|
0.2 ± 0.1
|
0.4 ± 0.1 (p = 0.51)
|
0.3 ± 0.1 (a: p = 0.01; b: p = 0.86)
|
|
Coronary artery diameter (µm)
|
27.3 ± 2.0
|
36.6 ± 2.8 (p = 0.008)
|
30.8 ± 3.0 (a: p = 0.32; b: p = 0.16)
|
|
Muscle function and remodeling
|
|
EDL max. force (N/cm2)
|
25.8 ± 5.7
|
28.8 ± 4.7 (p = 0.20)
|
28.1 ± 6.6 (a: p = 0.30; b: p = 0.9)
|
|
EDL fiber diameter (µm)
|
40.7 ± 1.7
|
35.3 ± 1.6 (p = 0.04)
|
37.1 ± 1.4 (a: p = 0.16, b: p = 0.79)
|
|
EDL slow fiber proportion (%)
|
2.25 ± 0.41
|
1.81 ± 0.48 (p = 0.78)
|
2.63 ± 1.12 (a: p = 0.62; b: p = 0.53)
|
|
EDL fast fiber proportion (%)
|
92.7 ± 1.1
|
86.6 ± 07.2 (p = 0.32)
|
93.1 ± 4.6 (a: p = 0.84; b: p = 021)
|
|
Soleus fiber diameter (µm)
|
44.3 ± 1.9
|
45.8 ± 1.5 (p = 0.74)
|
37.8 ± 1.5 (a: p = 0.01; b: p = 0.002)
|
|
Soleus slow fiber proportion (%)
|
30.3 ± 3.0
|
33.6 ± 8.5 (p = 0.73)
|
29.7 ± 2.6 (a: p = 0.87; b: p = 0.78)
|
