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Fig. 4 | Biological Research

Fig. 4

From: miR-146a-5p-mediated suppression on trophoblast cell progression and epithelial-mesenchymal transition in preeclampsia

Fig. 4

miR-146a-5p targets Wnt2. Note: The binding sites of miR-146a-5p in the 3′UTR of Wnt2 were predicted by StarBase and the corresponding mutated target sites were designed (A). Following transfection of miR-146a-5p mimic, miR-146a-5p inhibitor, mimic NC or inhibitor NC into HTR-8 cells, the mRNA and protein levels of Wnt2 in HTR-8 cells were quantified by RT-qPCR and Western blotting (B, C). HEK-293 T cells were cotransfected with miR-146a-5p mimic or miR-146a-5p inhibitor and MUT-Wnt2 or WT-Wnt2, and the luciferase activity was determined by dual luciferase reporter assay (D). All data are presented as mean  ±  SDs; n  =  3, **P  <  0.01. SD standard deviation; 3′UTR 3′untranslated region

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