Fig. 3

Wnt2 promotes the proliferation, migration, invasion and EMT of trophoblast cells. Note: pcDNA3.1-Wnt2, sh-Wnt2, pcDNA3.1 or sh-NC was transfected into HTR-8 cells, and then Wnt2 expression was determined by RT-qPCR (A) and Western blotting (B); the proliferative capacity of HTR-8 cells as measured by MTT assay (C) and colony formation assay (D); Transwell invasion assay (E) and Scratch assay (F) assessed the migratory capacity and invasiveness of HTR-8 cells separately; RT-qPCR (G) and Western blot (H) analyses for EMT-related proteins (Vimentin, N-cadherin and E-cadherin). All data are presented as mean  ±  SDs; n  =  3, *P  <  0.05, **P  <  0.01, ***P  <  0.001. SD standard deviation; EMT epithelial-mesenchymal transition