Fig. 6

T-96 enhanced sensitivity of human CaP cells to cisplatin. A T-96 enhanced the cytotoxic effect of cisplatin against CaP cells. DU145 and PC3 cells were treated with indicated concentrations of T-96 and cisplatin for 48 h. MTT was conducted to assess the effect of T-96 on cisplatin-induced cytotoxicity in CaP cells. B, C Combined T-96 and cisplatin treatment induced a higher rate of apoptosis than that of monotherapy. DU145 and PC3 cells were treated with T-96, cisplatin, and T-96 combined with cisplatin for 48 h, and then flow cytometry was used to assess the level of apoptosis-induced cell death. The expression levels of indicated proteins were detected using western blot. D T-96 and cisplatin co-treatment was more effective in initiating oxidative stress response and UPR. Western blot was used to examine the levels of NRF2 and the UPR associated proteins after treatment with T-96 combined with cisplatin. β-Tubulin was used as a loading control. Data represent the mean ± SD of three independent experiments. **P < 0.01 compared with the control group