Fig. 3

T-96 induced caspase-3-dependent apoptotic cell death in human CaP cells. A, C Apoptotic cell death in DU145 and PC3 cells was assessed using flow cytometry after treatment with T-96, Z-VAD-FMK (50 µM), or T-96 in combination with Z-VAD-FMK (50 µM) for 48 h. B The expression of indicated proteins in DU145 and PC3 cells were examined using western blotting after exposure to T-96 for 48 h. β-Tubulin was used as the loading control. Data represents the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001