Fig. 2

T-96 exhibited anticancer effects by inducing cell cycle arrest at the S phase. A T-96 suppressed the proliferation of CaP cells. EdU-staining assay was conducted to access the effect of T-96 treatment for 48 h on cell proliferation. Scale bar, 100 μm. B Flow cytometry was used to quantify the proportion of DU145 and PC3 cells in the different stages of the cell cycle in the presence of vehicle or T-96. The percentages of cell population in different conditions were quantified using three independent experiments. C Effects of T-96 on the expression levels of S-phase related proteins in DU145 and PC3 cells were examined using western blot after exposure to T-96 for 48 h. β-Tubulin was used as a loading control. All data are presented as the mean ± SD of three independent experiments. “*” represents P < 0.05; “**” represents P < 0.01; “***” represents P < 0.001