Fig. 1

The anti-proliferative effect of T-96 in human CaP lines. A Cell morphology of DU145 and PC3 cells after treatment with vehicle (0.05% DMSO) or the indicated concentrations of T-96 for 48 h as captured with a microscope. Scale bar, 100 μm. The histogram shows quantification of the cell proliferation rate. B Colony formation assay was used to evaluate the in vitro growth of DU145 and PC3 cells after exposure to the indicated doses of T-96 for 7 days. The colonies were captured as images and the numbers presented using a histogram. All data are presented as the mean ± SD of three independent experiments. *P < 0.05, **P < 0.01 and ***P < 0.001 versus vehicle