Fig. 6From: DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy miceDNMT1 overexpression reversed the impact of PPARα overexpression on apoptosis and ROS levels in HG-treated HRCPs. HRCPs were co-transfected with pCEP4-PPARα, pCEP4-NC and pcDNA3.1-DNMT1 or pcDNA3.1-NC, and then treated with 30 mM glucose. QRT-PCR (A) and WB (B, C) were performed to assess PPARα gene and protein expression in HRCPs. D, E Flow cytometry was performed to estimate apoptosis of HRCPs. F, G The levels of ROS in HRCPs was detected by DCFH-DA fluorescent probe. *P < 0.05 vs. Vector1; #P < 0.05 vs. PPARα + vector2Back to article page