Fig. 5From: DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy micePPARα overexpression repressed apoptosis and ROS levels in HG-treated HRCPs. HRCPs were transfected with pCEP4-PPARα, pCEP4-NC, si-PPARα (si-PPARα-1 and si-PPARα-2) or siRNA. A, B QRT-PCR was performed to assess the expression of PPARα in HRCPs. The modified HRCPs were treated with 30 mM glucose. C, D Flow cytometry was performed to estimate apoptosis of HRCPs. E, F The levels of ROS in HRCPs was detected by DCFH-DA fluorescent probe. *P < 0.05, **P < 0.01 vs. Vector; &P < 0.05, &&P < 0.01 vs. siRNABack to article page